Journal: The Journal of Neuroscience
Article Title: Perineuronal Nets Regulate the Inhibitory Perisomatic Input onto Parvalbumin Interneurons and γ Activity in the Prefrontal Cortex
doi: 10.1523/JNEUROSCI.0291-20.2020
Figure Lengend Snippet: List of primary and secondary antibodies used
Article Snippet: Sections were finally mounted on slides, dried for 12 h at room temperature, dehydrated with ascending alcohols, and rinsed in xylene before being coverslipped using Eukitt mounting medium (Sigma-Aldrich; 03989). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Primary antibodies Anti Host Isotype Dilution Company Antibody ID AnkG Mouse Monoclonal IgG1 1:500 Santa Cruz AB_626674 CamkII Mouse Monoclonal IgG1 1:500 Abcam AB_447192 c-Fos Rabbit Polyclonal 1:500 Synaptic Systems AB_2231974 FosB Mouse Monoclonal IgG1 1:500 Santa Cruz AB_783431 PV Guinea pig Polyclonal 1:2000 Synaptic Systems AB_2156476 PV Rabbit Monoclonal 1:2000 Swant AB_10000344 SYN Rabbit Polyclonal 1:1000 Millipore AB_570874 VGAT Rabbit Polyclonal 1:500 Synaptic Systems AB_887871 VGLUT1 Guinea pig Polyclonal 1:2000 Millipore AB_2301751 VGLUT2 Guinea pig Polyclonal 1:2000 Millipore AB_2187552 Secondary antibodies Anti Host Label Dilution Company Biotin A488 1:400 Invitrogen Guinea Pig Donkey A647 1:400 Invitrogen Guinea Pig Donkey A405 1:400 Invitrogen Mouse IgG1 Donkey DL549 1:400 Jackson Mouse IgG1 Donkey A488 1:400 Invitrogen Mouse IgG Donkey DL649 1:400 Jackson Mouse IgG Donkey A555 1:400 Molecular Probes Mouse IgM Donkey DL649 1:400 Jackson Mouse IgM Donkey DL549 1:400 Jackson Rabbit Donkey A405 1:400 Invitrogen Open in a separate window List of primary and secondary antibodies used Confocal microscopy and image analysis Sections were analyzed under a confocal microscope (Leica SPE).
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